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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Verification of Biomarker Candidates for High-Risk Diabetes Mellitus

2018년 8월 30일 16시 47분 32초
ANAL.P-410 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 19일 (금요일) 11:00~12:30
Analytical Chemistry
저자 및
Jiwon Ha, Seunghoon Back, Sang-Won Lee*
Center for Proteogenome Reserch, Department of Chemistry, Korea University, Korea
Diabetes mellitus (DM) is a common chronic disease that can cause various complications. Recently, there has been sharply increased in diabetes patients in Korea because of lifestyle change. When high-risk groups are diagnosed in early stage, immediate and suitable treatments can prevent the DM. However, accurate diagnosis in early stage of DM remains difficult due to its symptomless characteristic. Especially, Korean (Asian) have different risk factor for DM from European so that Korean specialized method is needed. We performed experiment using DM high-risk patient samples which were tracked past 10 years for discover DM early diagnosis biomarker specialized to Korean. Our research aims to identify the biomarkers that can predict whether high-risk patients would maintain their condition or develop to DM. Using Q-Exacitve mass Spectrometer, parallel reaction monitoring (PRM) was performed on the 80 patient digested serum samples to quantify 84 biomarker candidate proteins which are based on global profiling result. For sensitive and accurate quantification, 100 cm length long column and 200 min LC gradient were used in experiment. Stable isotope labeled peptides (, or AQUA peptides) were used to validate the targets identified in global profiling results and verify the expression changes of the targets.