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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Genome editing using CRISPR-Cas system

2018년 9월 1일 00시 15분 29초
LIFE2-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 00분
Life Chemistry - Recent Trends in Genome Editing Technique
저자 및
Sangsu Bae
Department of Chemistry, Hanyang University, Korea
Genome editing with engineered nucleases such as ZFNs (zinc finger nucleases), TALENs (transcription-activator-like effector nucleases), and CRISPR-Cas9/Cpf1 derived RNA-guided endonucleases is broadly used for biomedical research, biotechnology, and medicine. In addition, CRISPR base editors that enable the direct conversion of DNA bases without producing double-stranded breaks (DSBs) of DNA were developed. Unlike ZFNs and TALENs whose DNA specificities are determined by DNA-binding proteins, CRISPR nucleases use complementary base pairing to recognize target sites. Now, CRISPR nucleases are widely exploited due to the ease of use and inexpensive cost; researchers can induce gene editing at different sites by simply altering the guide RNAs. However, CRISPR nucleases cleave not only on-target sites but also off-target sites that differ by up to several nucleotides from the on-target sites, causing unwanted off-target mutations and chromosomal rearrangements. Here I present web-based programs, named CRISPR RGEN Tools (www.rgenome.net), including a novel CRISPR design tool and a genome editing assessment tool. These tools are indispensable for gene mutation in human cells, animals and plants. Furthermore, I would like to introduce versatile applications of CRISPR nucleases such as a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR, a circulating tumor DNA detection and the detailed mechanism of Cas9/Cpf1 revealed by single-molecule fluorescence imaging.