KCS

Recently, quantitative real-time PCR (QPCR) have been extensively used for the diagnosis of a wide range of infectious diseases. This technique found valuable clinical application in the diagnosis of viral diseases. However, QPCR suffers from several problems including time-consuming process and high fluorescent background signal. In the present work, we developed a novel molecular diagnostic method using SERS-PCR for the sensitive and specific detection of target genes. Due to the characteristics of amplifying Raman signals by factors up to 10-14 orders of magnitude and unique molecular fingerprinting information, surface-enhanced Raman scattering (SERS)-based biosensors provide ultrahigh sensitivity and have more advantages over the fluorescent detection method. In the present study, we employed an asymmetric-PCR to amplify the target gene in a short time, and then a SERS detection technique has been used for its highly sensitive detection. By combining the PCR and SERS as a detection tool, it would prove to be effective for the rapid and sensitive detection of a low concentration of target. References 1. Kneipp, K., Wang, Y., Kneipp, H., Perelman, L. T., & Itzkan, I, Phys. Rev. Lett. 78, 1667 (1997). 2. Wu, Y., Jiang, T., Wu, Z., & Yu, R, Biosens. Bioelectron, 99, 646-652 (2018). Keywords: SERS-PCR, Pseudomonas aeruginosa, Ratiometirc gene detection, molecular diagnostics