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제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Analysis of characteristic serum lipids of post-hepatectomy liver failure from partially hepatectomized swine using nanoflow UHPLC-ESI-MS/MS

2018년 8월 17일 10시 45분 58초
ANAL2.O-18 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 45분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
HaeA Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Since liver possesses the big capacity to regenerate, hepatectomy is a common treatment for the disease like hepatocellular carcinoma. However, liver resections are complex operations and have a risk of complication. Especially, post-hepatectomy liver failure (PHLF) which is a liver failure after operation is one of the most serious complications and a big part of the reason of death. Liver has an important role in lipid metabolism. Therefore if liver doesn’t function properly, the homeostasis of lipids might be failed. In this study, the changes of lipid profiles of swine serums were observed depending on rate of hepatectomy and time after operation using nanoflow ultrahigh-pressure liquid chromatography electrospray ionization tandem mass spectrometry (nUHPLC-ESI-MS/MS) to give more accurate insight into PHLF. The swines were divided into fake operation group (sham, n=6), 70% partial hepatectomy group (70% PHx, n=7), and 90% partial hepatectomy group (90% PHx, n=7). The serums were collected before operation, and 14 hours, 30 hours, and 48 hours after operation from each groups. The extracted lipids were structurally identified based on collision-induced dissociation (CID) experiments and individual serums were quantitatively analyzed through selective reaction monitoring (SRM) mode. The identified lipids were 142 phospholipids, 23 sphingolipids, and 240 glycerolipids.