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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Design of truncated-IK protein’s derivatives that alleviate inflammation

2018년 8월 17일 11시 59분 12초
ANAL2.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 30분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Hyunjun Jang, Ji Sun Kim , Yongae Kim*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
Rheumatoid arthritis is a chronic inflammatory disease. In Rheumatoid arthritis, activated immune cells attack the synovial membrane, cartilage and bone of the joints. So, the synovial membrane is abnormally proliferated and the bones and joints are destroyed. Although there are many causes for the disease, it is known that self-antigen recognition through abnormal MHC class II-expressing B cells excessively produces antibodies. Recent studies have shown that inhibitor K562 leukemic cell has been isolated and purified from the conditioned culture medium. This truncated IK (tIK) downregulates MHC class II on activation in inflammatory diseases. In our study, we examined the phosphorylation pattern of protein cell signaling by isolating macrophages from transgenic mice transplanted with the tIK nucleotide sequence, and found that tIK protein had the same effect as the anti-inflammatory cytokine IL-10. Therefore, we focused on the process of finding derivatives that are shorter and better anti-inflammatory than the previously reported tIK protien. We predicted the possible structure of tIK based on IL-10 through sequence homology modeling and could derive an epitope associated with anti-inflammatory activity. Based on these results, we proposed 4 anti-inflammatory peptide candidates and identified the anti-inflammatory activity through the TH17 cell differentiation test. Among them, the 18-mer peptide with anti-inflammatory activity was named tIK-YK4 and the short derivatives 9-mer and 14-mer peptides were also designed. Currently, we have successfully performed overexpression using E. coli and are optimizing the purification process. We are confirming peptide candidates using various techniques such as PAGE, CD, MASS.