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학술발표회초록보기

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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Multi-channel Microchip Electrophoresis for Rapid Screening of Glutathione S-transferase Polymorphism Genotyping

등록일
2018년 8월 23일 14시 13분 32초
접수번호
2611
발표코드
ANAL2.O-10 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 05분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Yucheng Sun, Seong Ho Kang1,*
Department of Chemistry, Kyung Hee University, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
A voltage programming (VP)-based microchip electrophoresis (ME) method was developed to detect specific sizes of DNA fragments. The optimum condition was deliberated on a single-channel microchip and was then applied to a multi-channel microchip for simultaneous high-throughput detection. Glutathione S-transferase (GST) polymorphism genes (M1/M1 = 215 bp/215 bp, M1/T1 = 215 bp/480 bp, and T1/T1 = 480 bp/480 bp) extracted from human blood were amplified by polymerase chain reaction and were introduced into the multi-channel microchip. Target DNA molecules amplified by only 10 PCR cycles could be detected by the VP-based multi-channel ME, but not by slab gel electrophoresis. Besides, the migration time for ME was less than 15 s which was 700 times faster than SGE. Furthermore, internal control (312 bp) was added for further identification of target DNA. The presented modality was demonstrated to be effective and rapid for highly sensitive and high-throughput screening of GST genes.

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