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제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Affinity-based Purification of Biopharmaceuticals Using Synthetic Binding Pairs

등록일
2019년 2월 13일 09시 10분 27초
접수번호
4017
발표코드
MAT.P-481 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 19일 (금요일) 11:00~12:30
발표형식
포스터
발표분야
Material Chemistry
저자 및
공동저자
An Jaeyeon, Junghyun Kim1, Kyeng Min Park1,*, Kimoon Kim*
Department of Chemistry, Pohang University of Science and Technology, Korea
1Center for Self-assembly and Complexity, Institute for Basic Science, Korea
Biopharmaceuticals including monoclonal antibodies (mAb) and cytokines have occupied a major class of pharmaceuticals because of their remarkable efficacy compared to chemical drugs. Genetic engineering significantly contributed to high-level expression of therapeutic proteins in mammalian cells and bacteria for the mass production. However, multi-step purification through various chromatographies, mostly due to inefficient isolation of proteins of interest, reduces the efficiency of biomanufacturing. Although protein A-based affinity chromatography has been used for mAb purification, additional chromatographies are typically required for removal of undesired proteins having similar structural properties with the target mAb. In addition, this affinity chromatography cannot be used for other proteins including cytokines which do not have Fc region of immunoglobulins for protein A interaction. Here we report an efficient affinity-based purification method using synthetic binding pairs. The interaction between the binding pair is not affected by biomolecules such as proteins with sufficient enough binding affinity. Thus, this synthetic binding pair system can be a good alternative for purification of therapeutic proteins.

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