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제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Parallel Reaction Monitoring to Verify Biomarker Candidates for High-Risk Diabetes Mellitus

등록일
2019년 2월 13일 09시 34분 54초
접수번호
4018
발표코드
ANAL.P-289 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 19일 (금요일) 11:00~12:30
발표형식
포스터
발표분야
Analytical Chemistry
저자 및
공동저자
Jiwon Ha, Seunghoon Back, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
Diabetes mellitus (DM) is a worldwide common chronic disease that can cause various complications. If patients of high DM risk were diagnosed in early stage, immediate and suitable treatments can prevent the DM development. Early diagnosis, however, remains difficult due to its symptomless characteristic of DM. Especially, Korean (or Asian) were known to have different patterns of risk factors for DM from those of European so that Korean specific diagnosis method is needed. We performed experiment using DM high-risk patient blood samples which were prospectively collected over past 10 years. We aimed to identify the biomarkers that can predict whether high-risk patients would maintain their condition or develop to DM. Using high resolution Q-Exacitve mass spectrometer, parallel reaction monitoring (PRM) was performed on the 80 patient digested serum samples to quantify 84 biomarker candidate proteins which are based on global profiling result. For sensitive and accurate quantification, 100 cm length long C18 column and 200 min LC gradient were used in experiment. Stable isotope labeled (SIL) peptides were used to validate the candidates identified in discovery experiments, resulting in a set of verified candidates for early diagnosis of DM.

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