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제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Ratiometric Two-Photon Fluorescent Probe for Monitoring of γ-Glutamyltransferase Enzyme

2019년 2월 14일 14시 19분 56초
ORGN.P-333 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 18일 (목요일) 11:00~12:30
Organic Chemistry
저자 및
Jae Hyung Jo, Hwan Myung Kim*
Department of Energy Systems Research, Ajou University, Korea
γ-Glutamyltransferase (GGT) is a cell-membrane-bound enzyme, which selectively hydrolyzes the cleavage of the γ-glutamyl bond in glutathione (GSH). It is found that GGT plays an important role in cellular GSH and cysteine homeostasis, involving in diverse physiological and pathological processes. Actually, it has been discovered that overexpressed levels of GGT are connected with tumorigenesis in several human cancer cell, including ovarian and colon cancer. Therefore, GGT has been recognized as a potential biomarker of malignant tumors. In this respect, there is growing interest in fluorescent probes for GGT and several GGT activatable fluorescent probes have been developed for monitoring of GGT both in tumor cells and living animals. However, only a handful of one-photon ratiometric fluorescent probe is known that is suitable for biological imaging. In this work, we developed a new ratiometric two-photon fluorescent probe for GGT by incorporating the γ-glu-substrate and indocyanine fluorophore. This probe showed both one-and two-photon excited fluorescence and large stokes shift as well as high cell loading ability. The probe was readily activated by GGT and successfully applied for one- and two-photon microscopy imaging in live cells.