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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Effect of aging on lipidomic changes in mouse serum, kidney, and heart

등록일
2019년 2월 7일 15시 14분 17초
접수번호
5002
발표코드
ANAL1.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 09시 : 56분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
Jung Yong Eum, JongCheol Lee1, Myeong Hee Moon1,*
Yonsei University, Korea
1Department of Chemistry, Yonsei University, Korea
Aging is defined as the gradual decline of metabolic functions and is becoming the global burden due to the extended human life expectancy. The detailed metabolism of aging is still not fully understood, but it is well known that aging causes intracellular accumulation of reactive oxygen species which could develop age-related diseases such as cardiovascular disease, neurodegenerative disease, and diabetes mellitus. Since changes in lipid metabolism may induce these diseases, examining the alteration of lipid levels is important. In this study, using serum, kidney, and heart from the 4- and 25-month-old C57BL6 female mice, a comprehensive lipidomic analysis was done to elucidate age-induced perturbation of lipid profiles by nanoflow ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (nUHPLC-ESI-MS/MS). Among 542 identified lipid species, 279 lipid species were quantified in selected reaction monitoring mode. While most of the lipid species with significant changes (> 2-fold, p < 0.01) were found to be decreased by aging, triacylglycerols were increased in mitochondria rich tissues, especially in the heart. The ratio of phosphatidylcholine over phosphatidylethanolamine (PC/PE) which was the important factor of mitochondria membrane integrity was found to be significantly decreased only in kidney. This presentation demonstrates that nUHPLC-ESI-MS/MS analysis can determine the lipid species showing significant changes in serum, kidney, and heart of aged mice.

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