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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Optimization of nanoflow ultrahigh performance liquid chromatography (nUHPLC) conditions for high-throughput and rapid lipid analysis by ESI-MS/MS

등록일
2019년 2월 7일 15시 32분 23초
접수번호
5003
발표코드
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발표시간
목 09시 : 52분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
Gwang Bin Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Since separation in the nanoflow ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (nUHPLC-ESI-MS/MS) is carried out at nanoflow rate regimes, performances of separation can be improved with the increased resolution with the minimization of sample amounts to be injected and the consumption of organic solvents. However, when a large number of sample analysis is required, a high speed separation is necessary for nUHPLC compared to the typical gradient elution condition that requires 30 min for a separation of lipids with broad classes without incurring spectral congestion in MS detection. This study demonstrated that high-throughput and rapid lipid analysis can be carried out within 10 minutes of separation by adjusting gradient elution condition with the increase of flowrates but without increasing the spectral congestion. The average peak width can be decreased to 0.14 min for PC 16:0/16:0 in a 10 min run condition compared to 0.15 min for a 30 min run condition with the standard deviation of retention time in repeated measurements as 1.68 s. The plate numbers of PC 16:0/16:0 can be above 330,000. In addition, MS detection efficiencies of lipid species upon increasing separation speed was evaluated with lipid standards spiked to a serum sample.

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