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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Investigation of changes in serum lipid levels upon the partial hepatectomy of swine in relation to the post-hepatectomy liver failure using nanoflow UHPLC-ESI-MS/MS

2019년 2월 7일 15시 46분 44초
ANAL1.O-13 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 48분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
HaeA Kim, JongCheol Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Post-hepatectomy liver failure (PHLF) is the inability of the liver to perform its normal synthetic and metabolic function after liver resection to treat diseases like hepatocarcinoma. Despite much progress in hepatectomy, PHLF still has high morbidity and mortality. Therefore, PHLF is still a serious complication and a possible diagnostic method in its early development stage after surgery is required to be developed. In this experiment, the changes in serum lipid levels of swine serum were investigated according to the degree of liver resection and elapsed periods after operation using nanoflow ultrahigh-pressure liquid chromatography electrospray ionization tandem mass spectrometry (nUHPLC-ESI-MS/MS). Serum samples were collected at four time intervals: before the operation, after 14 hours, 30 hours, and 48 hours of partial hepatectomy operation from six sham swine which did an only laparotomy, seven sines with 70% partial hepatectomy (70% PHx), and seven PHLF swines (90% PHx) which underwent 90% partial hepatectomy. A total of 151 phospholipids, 22 sphingolipids, and 204 glycerolipids were structurally identified based on collision-induced dissociation (CID) experiments and each lipids species of individual serum sample was quantitatively analyzed using selective reaction monitoring (SRM) mode. The alterations in lipid levels between 70% PHx group and 90% PHx groups were statistically evaluated to discover candidate marker species showing significant differences.