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제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A resistance study of serum-starvated neuroblatoma cell at specific drug concentration

2019년 2월 12일 12시 24분 55초
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목 09시 : 20분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Sooyeon Chae, Jong Yoon Han, Chae Eun Heo, Min Gyeongseo, MyungKook Son, Min Ji Kim, Hugh I. Kim, Chae Ri Park, Dongjoon Im, Chaehyeon Yoon, Hugh I. Kim*
Department of Chemistry, Korea University, Korea
Neuroblastoma is a solid tumor that arises from the developing sympathetic nervous system. As neuroblastoma belongs to heterogeneous disease, designing effective treatment for each patient is challenging. To examine individual variation in drug response, drug stability and efficiency tests are commonly performed in 2-dimensional (2D) cell or xenograft before clinical trial. However, additional condition to in vitro systems is crucial to closely mimic the complexity of in vivo system. Serum starvation is one of the most frequently used methods to imitate in vivo microenvironment. Thus, examining drug efficacy and resistance of serum-starved 2D cell has been suggested as a screening strategy for individual therapy. However, the differences between serum-starved and normal cells have not been fully understood. In this study, we studied the drug resistance in cancer cell depending on serum starvation. Especially, Topotecan, one of the anticancer drugs which inhibit DNA synthesis, was used. We observed that neuroblastoma cells in serum starvation show the resistance when they are treated with 1 μM topotecan. Then, quantitation of drug uptake using reverse phase liquid chromatography-triple quadrupole mass spectrometry were performed. As a result of the quantitation of drug uptake, Serum starvation conditions showed relatively lower uptake at 48 hours when chemoresistance occurs. In correlation to distinct change in cellular uptake, we also performed the proteomic analysis to find differentiated protein group related to resistance in serum starvation. The fundamental understanding of anticancer drugs resistance on serum starvation can provide prediction of strategy for individual therapy.