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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Novel zwitterionic fluorescent polythiophene sensor for E.coli. detection

등록일
2019년 2월 13일 12시 11분 39초
접수번호
5020
발표코드
ANAL1.O-21 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 10시 : 20분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
Jimin Shim, Salah Mahmoud Tawfik Ahmed, Yong-ill Lee*
Department of Chemistry, Changwon National University, Korea
We developed a novel zwitterionic fluorescent polythiophene with high sensitivity for the detection of gram-negative pathogenic bacteria. The sensing probe using the zwitterionic fluorescent polymer was constructed with three different water soluble thiophene monomers via oxidative polymerization reaction. The zwitterionic fluorescent polythiophene bound to bacteria through ionic complexes between anionic groups on the bacterial surface and cationic groups after 15 min incubation. This finding demonstrated that the fluorescence on system operated via changes in the hydrophilic and hydrophobic nature of the zwitterionic fluorescent polymer at pH (7.4), at a fixed polymer concentration. The limit of detection for Escherichia coli was found to be 0.033 cfu/mL with linear range from 1.15x108- 1.15x109 cfu/mL. With these encouraging results, it is expected that it would open revenues for promising applications of zwitterionic polythiophene in real samples. Keywords : zwitterionic polythiophene, Gram-negative bacteria, E.coli, Fluorescence enhancement, Bacterial surface

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