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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 HPLC-UV Determiation of Resveratrol, Oxyresveratrol and Piceatannol from Wine

등록일
2019년 2월 8일 13시 54분 43초
접수번호
5107
발표코드
ANAL2.O-16 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 00분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Jongsung Noh, JinSol Lee, Won Hoe Koo, Hyun-Woo Cho1, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Department of Natural Science Chemistry, Kyonggi University, Korea
Polyphenols are phytochemicals that exist in various plants and are beneficial to human health. These ingredients prevent aging, protect the skin from ultraviolet rays, improve brain health and prevent dementia. Resveratrol, oxyresveratrol and piceatannol in grapes are typical polyphenols, and grape wine also contains these ingredients. In this study, resveratrol, oxyresveratrol and piceatannol in wine were extracted by deep eutectic solvent based dispersive liquid-liquid microextraction (DES-DLLME) and a method for quantification these polyphenols using high performance liquid chromatography - UV/vis spectroscopy was established. Parameters for sample clean up and concentration were optimized and verified. Experiments were carried out to obtain optimal parameters such as the types and volumes of extraction solvent and dispersive solvent, volume of acetic, anhydride, time of derivatization, type of basic solution for derivatization, and the effect of salt. Chromatographic separation with HPLC/UV-vis was performed using the Innoval ODS-2 (4.6 mm id × 150 mm length, 5 μm particle size) column and gradient elution mode using water and methanol.

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