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  • 02월 28일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제123회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Infrared spectroscopic characterization of bile juices acquired from patients with gallbladder diseases

2019년 2월 9일 13시 23분 01초
ANAL2.O-11 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 40분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Eunjin Jang, Hoeil Chung1,*
Chemistry, Hanyang University, Korea
1Department of Chemistry, Hanyang University, Korea
Bile juice is a body fluid related with emulsifying and micellizing cholesterol in intestine, secreted in liver and stored in gallbladder (GB). Composition of bile juice would be different by health conditions of each patient; therefore, bile juice could be a beneficial medium for studying GB diseases. In this study, infrared (IR) spectroscopy has been used to study bile juices from patients of GB polyp and GB cancer. Before IR measurement, raw bile juice samples were pretreated with chloroform-methanol mixture solution to extract and separate organic, aqueous and protein agglomerates. Then, each phase-separated sample was dropped on hydrophobic OTS (octadecyltetrachlorosilane)-coated Si wafer to form reproducible circular droplet and dried for IR spectral acquisition. IR spectra were collected across a dried sample (line-mapping) and a representative spectrum was selected among the mapped spectra for further examination. In the chosen spectra, key spectral features related with GB polyp and GB cancer were searched and characterized. Next, to examine potential of IR spectroscopic discrimination of bile extracts according to diseases, principal component analysis (PCA) was used to reduce dimensions of spectral data into simply 2- or 3-dimensional score domains to easily recognize potential differences between GB polyp and cancer samples. In final, the future direction of this research will be discussed.