KCS

The important of adequate amounts antibiotic use can’t be overestimated. Due to the abuse of classical antibiotics, antibiotic resistance has been increasing. So studies of cationic amphipathic peptides come into the spotlight as a new antibiotic. And there is still the potential for being used as candidate of good antimicrobial agent. It is also indispensable to discover new compounds to counteract the existing antibiotic resistant bacteria in the world Lactophoricin is a cationic amphipathic peptide with 23-maino acid residues derived from bovine milk. And we developed another new design to develop other novel AMPs with a more effective and less toxic antimicrobial using the sequence of the LPcin-YK3 peptide. Antimicrobial activity of designed novel AMP analogs was confirmed by bacterial killing assays and growth inhibition assays for bacteria and fungi, and the stability was confirmed by hemolysis assay and cytotoxicity assay for mouse and human eukaryotic cells. In order to understand the relationship between antibiotic function and structure of AMPs we conducted structural studies of novel AMPs using various spectroscopic methods and NMR. We successfully expressed of LPcin in E. coli and purified from the cell extract using various biophysical techniques. To confirm their 3D structures in membrane environments, we conducted solid-state NMR.