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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Condition in which nanodiscs produced by SMA(styrene:maleic acid)copolymer reliably change the orientation by Ytterbium(Yb3+)

2019년 7월 29일 14시 10분 06초
PHYS.P-130 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 17일 (목요일) 11:00~12:30
Physical Chemistry
저자 및
Jae-Woong Kim, Dong-Kuk Lee1,*
Fine Chemistry, Seoul National University of Science & Technology, Korea
1Department of Fine Chemistry, Seoul National University of Science & Technology, Korea
In general, bicelle(DMPC/DHPC) is used for structural analysis of membrane proteins using nuclear magnetic resonance(NMR). Because bicelles exist only in certain orientation by the magnetic field. However, surfactants are used to fix membrane proteins of cell membrane in the bicelles. The structure of proteins can be denatured by surfactants. Polymer-based nanodiscs were developed to solve these problems. Polymer-based nanodiscs can directly extract membrane proteins from cell membranes. In this study, we conducted experiments with nanodiscs based on SMA(styrene:maleic acid)copolymer. When structural analysis is performed using solid-state nuclear magnetic resonance(ssNMR), information on at least two orientations is required to interpret the three-dimensional structure. When Ytterbium(Yb3+) is added, the direction of nanodiscs is turned at 90°. Experiments were conducted to find suitable conditions for nanodiscs produced by SMA-based polymers to reliably change the orientation under Yb3+ presence. Through ssNMR analysis, information on the orientation of polymer-based nanodiscs was obtained. Nanodiscs produced by SMA-EA are negatively charged, causing them to clump as the concentration of Yb3+ increases. EDTA4- was used to address these issues, but not dramatically. Nanodiscs produced by SMA-QA are positively charged, so the orientation was changed reliably by Yb3+.