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대한화학회 제124회 학술발표회 및 총회 Diffusion Enhancement of Catalytic Enzymes

등록일
2019년 8월 19일 11시 42분 17초
접수번호
0360
발표코드
KCS4-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
수 15시 : 00분
발표형식
심포지엄
발표분야
KCS - [IBS Symposium] Dynamic Soft and Living Matter
저자 및
공동저자
Ah-Young Jee, Steve Granick*
Center for Soft and Living Matter, Institute for Basic Science, Korea
Too much remains speculative about how the internal dynamics of enzymes relates to catalytic function, due to lack of appropriate methods to detect millisecond time scales over sufficiently small observation windows. Here, we used super-resolution microscopy (stimulated emission-depletion fluorescence correlation spectroscopy, STED-FCS) and observe than during catalysis an active enzyme spontaneously migrate toward the direction of lower reactant (substrate) concentration using the run-and-tumble motion, a strategy similar to that of some swimming microorganisms. Experimentally, we show that enhanced enzyme mobility is generated by super-diffusive kicks generated at the substrate turnover frequency. Pursuing this idea, we have explored methods to vary systematically the turnover frequency, kcat, which defines how many substrate molecules are consumed in a given time. These results help us understand activities of enzymes, and furthermore to design and use a synthetic self-propelled swimmer relevant to "active matter".
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