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대한화학회 제124회 학술발표회 및 총회 Label-free Mass Spectrometric Imaging for Biological Applications

등록일
2019년 8월 22일 14시 42분 58초
접수번호
1210
발표코드
LIFE2-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 35분
발표형식
심포지엄
발표분야
Life Chemistry - Light Irradiation Deep into Life-Science Chemistry
저자 및
공동저자
Tae Geol Lee
Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Korea
Recently, surface mass spectrometric analysis (and imaging) techniques such as MALDI-MS and ToF-SIMS are getting popular since they can measure chemical compositions/distributions of bio-related sample surfaces. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used for molecular identification of proteins in the discovery of disease-related biomarkers as a key platform technique in proteomics. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) on the other hand has been used to analyze small biomolecules such as amino acids, peptides, vitamins and drugs in the top-layer (1-2 nm probing depth) because the collisional cascade process cannot produce secondary ions with a molecular weight of over m/z 2,000 without the use of noble metals or MALDI matrixes. First, we will show our recent ToF-SIMS data of tissue samples obtained by using various primary ion beams and sampling methods, and discuss the potential capability of ToF-SIMS to study for –omics, particularly lipidomics for tissue study.[1-6] Second, we will show that ToF-SIMS imaging analysis of peptides enables efficient screening for enzyme inhibitors, thus permitting high-throughput drug screening with high accuracy, sensitivity and specificity.[7] Finally, a new strategy of biomolecules (microRNA and proteins) detection by using imaging mass spectrometry will be presented.[8] [1] Yun et al., Anal. Chem. 83, 9298-9305 (2011) [2] Park et al., Scientific Reports, 5:11077 (2015) [3] Shon et al., Biointerphases, 11, 02A321 (2016) [4] Son et al., J. Vac. Sci. Tech. B, 34, 03H133 (2016) [5] Kim et al., J. Am. Soc. Mass Spectrom. 28:1729-1732 (2017) [6] Shon et al., Biointerphases, 13, 03B411 (2018) [7] Cho et al., Anal. Chem. 89, 799-806 (2017) [8] Na et al., submitted