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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Isolation of single-chain variable fragments about Lysophosphatidic acid receptor 2 (LPA2) using purified recombinant LPA2 as the target

2019년 8월 22일 16시 59분 32초
LIFE.P-220 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (금요일) 11:00~12:30
Life Chemistry
저자 및
Ji Young Lee, Yeon Gyu Yu1,*
Kookmin University, Korea
1Department of Bionano Chemistry, Kookmin University, Korea
Lysophosphatidic acids (LPAs) are potent signaling molecules that influence diverse cellular responses such as cell proliferation, migration, cell survival, and pathological responses. LPA receptors belong to the G protein coupled receptor (GPCR) family, and seven subtypes of LPA receptors have been identified. Among them, LPA receptor 2 (LPA2) is involved in the proliferation and metastasis of ovarian and breast cancers. Hence, LPA2-specific antagonists or antibodies are considered as potential anticancer therapeutics. To develop antibodies against LPA2, the recombinant LPA2 was expressed in Escherichia coli and purified in homogeneous form. The purified LPA2 was stabilized with biotin conjugated amphiphilic polymers (biotin-APG). The stabilized LPA2 was immobilized on streptavidin magnetic beads or streptavidin coated immunoplates, and OPALS phage library displaying single-chain variable fragments (scFvs) was applied to select LPA2-specific scFv clones. After biopanning, a few scFv clones which showed specific binding to LPA2 were identified, and their binding properties to LPA2 had been characterized.