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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Exosome isolation from human serum using different centrifugation speeds

등록일
2019년 8월 22일 17시 34분 38초
접수번호
1559
발표코드
ANAL3-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 00분
발표형식
심포지엄
발표분야
Analytical Chemistry - Recent Trends in Mass Spectrometry
저자 및
공동저자
Jeongkwon Kim
Department of Chemistry, Chungnam National University, Korea
Exosomes are nanosized vesicles commonly found in biological fluids. Changes in the morphology and/or concentration of exosomes in the bloodstream can be related to specific disease stages and may be used to estimate cancer status. Exosome enrichment using ultracentrifugation at a speed of around 100,000xg is currently the most commonly used exosome enrichment method. In this study, the effect of different centrifugation speeds was investigated. The isolated exosomes were analyzed using the field emission-scanning electron microscope (FE-SEM), bicinchoninic acid assay (BCA assay), 1-D gel separation, western blotting, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FT-ICR MS). The size distribution and protein amounts of exosomes were confirmed by FE-SEM and BCA assay.

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