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  • 09월 11일 00시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제124회 학술발표회 및 총회 Optical Sensing and Control of Protein Activity in Live Cells by Fluorescent Protein Technology

등록일
2019년 8월 27일 17시 45분 50초
접수번호
1653
발표코드
LIFE2-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 09시 : 00분
발표형식
심포지엄
발표분야
Life Chemistry - Light Irradiation Deep into Life-Science Chemistry
저자 및
공동저자
Jihye Seong
Brain Research Institute, KIST, Korea
Recent advances in fluorescent protein (FP) technology made a great contribution to the development of current biology field. For example, florescent molecular biosensors based on fluorescence resonance energy transfer (FRET), which can be detected between donor and acceptor FPs in proximal, provide powerful tools to visualize dynamic molecular interactions and protein activity in live cells. I will discuss how the FRET-based kinase biosensors are developed to report the real-time kinase activity at subcellular membrane compartments. I will also introduce newly developed GPCR biosensors based on circular permuted FP (cpFP). Fluorescent protein technology can be further applied to control protein activity by light. In particular, I will introduce an optogenetic strategy to control protein activity by caging the functional domain of the target protein with photodissociable Dronpa dimmer. As the caged domain can be exposed as the dimeric Dronpa became monomers by photoswitching, we can control the protein activity by light. Taken together, fluorescent proteins and advanced imaging technologies enable both optical sensing and control of target protein activity in live cells, which will be powerful tools to understand the real-time molecular events with high spatiotemporal resolutions.
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