KCS

MS-based proteomics of 3D cell model and single cell system has been extremely successful for quantitation of proteins that are integral components of essential processes for life and continues to revolutionize protein characterization. Development in different areas of proteomic workflow such as sample preparation, MS instrumentation and data processing have enabled us to get comprehensive information from MS-based proteomics. However, exploring cellular heterogeneity in tumors continues to be a challenge among researchers using cell models. Development of 3D cells has increased our understanding of cellular heterogeneity in tumor cells, an achievement which has caused the shift of recent researches towards the use of 3D cell culture. In this study, we optimized 3D spheroids cell culture condition by adjusting 3D scaffold and incubation conditions. Furthermore, we performed a comparative study of 2D and 3D spheroids cells by evaluating differences in anti-cancer drugs toxicity in these cell models. Additionally, ESI-MS was employed to highlight further differences between 2D and 3D neuroblastoma cells by emphasizing on differences in drug uptake mechanisms of these 2 cell models. This study will enhance our understanding of 2D and 3D spheroid cell model impacts in optimizing the efficacy of anti-cancer drugs and will contribute to the development of pre-treatment methods of 2D and 3D spheroid model for MS-based proteomics.