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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Characterization of Structural Change of Protein during Gas Phase Transition Using Electrospray Ionization (ESI) with Fourier Transform-Infra red (FT-IR) spectrometer

등록일
2019년 8월 28일 14시 17분 53초
접수번호
1689
발표코드
ANAL.P-156 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 18일 (금요일) 11:00~12:30
발표형식
포스터 분석구두발표
발표분야
Analytical Chemistry
저자 및
공동저자
MyungKook Son, Tae-Won Ko, Chae Eun Heo, Chae Ri Park, Paul Valery Migisha Ntwali, Sooyeon Chae, Min Ji Kim, Hugh I. Kim*
Department of Chemistry, Korea University, Korea
Electrospray ionization (ESI), is a soft ionization technique that produce gas phase ions by applying high voltage to a sample solution. Solution samples are sprayed into air, creating gas-phase ions by evaporation process. ESI coupled with ion mobility mass spectrometry (IM-MS) has been widely used to study gas-phase ions, which produces information of gas-phase structures of the ions. Many researchers have been utilized ESI-IM-MS to study solution phase structure of proteins by relating with gas-phase structures. However, there is no evidence whether the conformations of proteins are intactly preserved during the evaporation process.. So, to observe the phase transition of protein ions from solution to gas phase, we built home built ESI coupled with Fourier transform-infra red (FT-IR) spectrometer. We firstly monitored lysozyme in deuterium oxide-tris buffer to see IR spectrum of the alpha helix peak of protein. Hopefully, the results of this study will help to elucidate how gas-phase protein ion conformations are formed, or if the gas-phase conformation is directly related to the solution-phase conformation of proteins.

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