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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A novel siRNA delivery complex using Cell-Penetrating Peptide (CPP)-Peptide Nucleic Acid (PNA) conjugates.

2019년 8월 28일 16시 32분 06초
LIFE.P-227 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (금요일) 11:00~12:30
Life Chemistry
저자 및
Prajwala Devarapalli, Yong Ho Kim1,*
Nanoscience and Technology, Sungkyunkwan University, Korea
1Department of Chemistry, SKKU Advanced Institute o, Korea
Effective and stable delivery of siRNA (small interfering RNA) into the cells require translocation of siRNA into the cytosol to treat multiple diseases. Several carriers have been employed to enhance the stability and efficiency of siRNA delivery. Considering their safety, efficiency, and targeting capabilities, cell-penetrating peptides can be the solution for overcoming the major obstacles in siRNA delivery. Another side, Peptide nucleic acid (PNAs) are DNA analogs, in comparison to nucleotides they have better chemical stability. In this study, we developed a novel delivery platform based on the CPP-PNA conjugates as a linker can be complexed with siRNA, where PNA called as a universal linker that is complementary to the part of siRNA but not sequence-specific. The complex formation was optimized by the stoichiometry of each component, confirmed by gel retardation assay. In vitro, the transduction efficiency of the complex was higher and cytotoxicity of the Peptide PNA conjugates did not show up to 20uM. The benefit of the system is not only cell penetration and allows better stability in serum environment to siRNA. Delivery efficiency and stability of the complex can expect high silencing activity. This new technology can greatly reduce difficulty and cost without a chemical modification and it can be an efficient approach for cell-specific delivery of siRNA.