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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics

등록일
2019년 8월 29일 14시 37분 42초
접수번호
1823
발표코드
ANAL3-4 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 14시 : 30분
발표형식
심포지엄
발표분야
Analytical Chemistry - Recent Trends in Mass Spectrometry
저자 및
공동저자
Jae Hun Jung, Jong-Seo Kim1,*
Department of ophthalmology, Yonsei University, Korea
1Seoul National University, Korea
The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide “diethylation” using only 13C isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based dimethylation labeling in both a singleshot and multidimensional LC−MS/MS analysis of the HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed diethylation outperformed isobaric labeling approaches in terms of the quantification accuracy or the number of protein identifications, generating more than two times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed diethylation method could contribute to various types of quantitative proteomics applications in which three of multiplexity would be sufficient.

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