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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Immobilization of lipid vesicle using His-tagged biocytin on a supported lipid bilayer.

등록일
2019년 8월 29일 15시 31분 42초
접수번호
1847
발표코드
PHYS.P-213 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 17일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Physical Chemistry
저자 및
공동저자
Sujin Lee, Hahkjoon Kim1,*
Department of Chemistry, Duksung Women’s University, Korea
1Department of Chemistry, Duksung Women's University, Korea
We developed a new scheme for surface attachment of lipid vesicle using His-tagged biocytin (His-biocytin) which is relatively easy to synthesize compared to the His-tagged lipid for single molecule study. We labeled supported lipid bilayers on a glass coverslip with His-biocytin and allowed nitrilotriacetic acid (NTA) containing lipid vesicle to bind to the membrane in the presence of Ni2+. We employed a confocal fluorescence microscope to observe the specific binding of NTA-lipid containing lipid vesicle on the His-biocytin labeled membrane. Unlike conventional vesicle attachment scheme using a streptavidin-biotin binding, this method uses a reversible metal-chelate binding to create a new surface. To apply this method to single molecule study, we measured fluorescence lifetimes of TMR biocytin-streptavidin complex in the lipid vesicle. We found that the protein-dye complex in the lipid vesicle rotates freely and does not adhered to the inner surface of the lipid vesicle based on the fluorescence lifetime of the TMR biocytin bound to streptavidin in the lipid vesicle.

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