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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제124회 학술발표회 및 총회 A highly sensitive and multiplexed target DNA detection strategy using DNA-modified magnetic microparticles

등록일
2019년 8월 29일 16시 54분 35초
접수번호
1894
발표코드
MAT.P-391 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 17일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Material Chemistry
저자 및
공동저자
Min Ji Hwang, Dongkwon Lim*
KU-KIST Graduate School of Converging Science and Technology, Korea University, Korea
Magnetic particles have recently received significant research attention owing to their unique magnetic properties and low toxicity. Here, we suggest the DNA-modified magnetic micro particles based strategy for sensitive and multiplexed target DNA detection. The amine-modified DNA was modified on the tosyl-modified magnetic micro particles (MMP) as a target capture probe sequence then, we designed the half-complementary signal probe with fluorescence molecules. The use of MMP facilitates the assay process after mixing with analyte containing possible target DNA sequence. Then, the fluorescence signal intensity can be measured with fluorometer. In this work, the method can simultaneously identify sequences bacteria such as Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The method showed femto molar sensitivity to bacterial target DNA without interference, which showed a strong potential to identify multiplexing different target bacteria.

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