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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Monitoring the changes of mitochondrial membrane potential using SERS in the live single cell

2019년 8월 29일 17시 00분 13초
ANAL.P-174 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (금요일) 11:00~12:30
Analytical Chemistry
저자 및
Ji Hye Lee, Dongkwon Lim*
KU-KIST Graduate School, Korea University, Korea
Obtaining the molecular information from inside cell in real time has been a critical issue to study the interaction between molecules of interest and intracellular components. Fluorescence-based technique has been an excellent tool to study such important interactions for years. We are demonstrating the Raman scattering based method as a promising platform to achieve the real-time monitoring of the subtle molecular changes occurred in inside cells. The triphenylphosphine-modified AuNPs were selectively localized in the mitochondria and showed the characteristic Raman spectrum of cytochrome C and other Raman spectrum of molecular components inside cell. The Raman scattering based method enabled to observe the changes of mitochondrial membrane potential in the single cell of rheumatoid arthritis synovial fibroblasts induced by protein (TNF-a), various chemicals (MgCl2, sodium pyruvate, FCCP), and non-chemical stimulus (i.e., light). The surface-enhanced Raman spectrum originated from mitochondria were sensitively changed with time when induced mitochondrial depolarization by the additions of TNF-a, or chemicals to induce the changes of mitochondrial membrane potential.