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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Supported Lipid Bilayer Platform for Macromolecular Detection Applications

2019년 9월 9일 15시 20분 55초
LIFE.P-230 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (금요일) 11:00~12:30
Life Chemistry
저자 및
Bo Kyeong Yoon, Joshua Alexander Jackman1,*
Chemical Engineering, Sungkyunkwan University, Korea
1Chemical Engineering, Sungkyunkwan University, United States
The supported lipid bilayer (SLB) platform is a versatile biomimetic lipid membrane that is widely used in chemical and biological sensing applications. To date, it has proven challenging to fabricate highly biomimetic SLBs with existing methods, e.g., vesicle fusion, because they only work with a limited range of lipid compositions and solid supports. In particular, there is growing interest to develop SLBs that contain high cholesterol fractions due to enhanced biomimetic character and durability. In this work, we developed a highly efficient protocol called the solvent-assisted lipid bilayer (SALB) method to fabricate high-quality SLBs. The SALB method allows cholesterol-rich SLBs to be prepared with tunable cholesterol fractions up to ~52 mol%. As an application example, we demonstrated that fatty acids and monoglyceride derivatives induce distinct membrane morphological responses in SLBs, as indicated by quartz crystal microbalance-dissipation (QCM-D) measurements. While fatty acid addition to SLBs induced tubule formation with a greater extent of membrane remodeling at higher cholesterol fractions, monoglyceride addition caused bud formation with a lower degree of membrane remodeling at higher cholesterol fractions. Altogether, our findings demonstrate how SLBs can function as highly sensitive detection platforms based on triggering specific membrane morphological responses and such capabilities along with other advantageous properties of SLB platforms can be useful for a wide range of medical and biotechnology applications such as cancer diagnostics.