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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Label-Free Electrochemical Detection of Protein Kinase A Activity with C-Kemptide-AuNP/rGO-GCE

등록일
2019년 9월 9일 16시 54분 42초
접수번호
2029
발표코드
ELEC.P-447 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 17일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Electrochemistry
저자 및
공동저자
Da eun Oh, Tae Hyun Kim1,*
Chemistry, Soonchunhyang University, Korea
1Department of Chemistry, Soonchunhyang University, Korea
Protein kinase A (PKA), playing a key role in the human biological system, especially involving in peptide phosphorylation and enzymatic signal amplification. PKA activity provides many information of some disease, such as cancer, leukaemia, HIV. Therefore, a lot of researchers have developed various methods to detect the activity of PKA sensitively and selectively. Here, we designed the label-free method to selectively detect the PKA activity with electrochemical impedance spectroscopy (EIS). The electrode for the EIS measurements was modified with gold nanoparticle/reduced graphene oxide (AuNP/rGO) hybrid nanocomposite to enhance the electrochemical catalytic effect. The AuNP/rGO hybrid nanocomposite was fabricated by simple and environmentally friendly method using electrochemical technique. For the PKA activity detection, the C-Kemptide was immobilized onto AuNP/rGO modified electrode (KAG-GCE) through gold-thiol bond. As-prepared KAG-GCE was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV), and EIS. Here, the Rct in EIS measurement was representing the change in PKA activity; the Rct increased with phosphorylation of C-Kemptide by PKA. So, we measured the PKA activity though changes in Rct according to the concentration of ATP and PKA. The inhibition assay was also performed with the H-89 kinase inhibitor. Furthermore, the KAG-GCE based PKA assay was applied to the ovarian cancer cell lysate. Our PKA assay showed a great potential for the use of actual clinical diagnosis.

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