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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Electrochemical Aptasensor for Detection of Pb2+-using Methylene Blue Tagged DNA and ERGO

등록일
2019년 9월 9일 16시 59분 26초
접수번호
2030
발표코드
ELEC.P-448 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 17일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Electrochemistry
저자 및
공동저자
Seungjoo Jang, Tae Hyun Kim1,*
chemistry, Soonchunhyang University, Korea
1Department of Chemistry, Soonchunhyang University, Korea
We have developed a sensitive aptasensor for the detection of Pb2+ ions based on G-quadruplex DNA and an electrochemically reduced graphene oxide (ERGO). Here, ERGO was coated on a glassy carbon electrode (GCE) surface by reduction of graphene oxide using cyclic voltammetry. After that, a methylene blue (MB)-tagged, guanine rich DNA aptamer (Apt) was attached on ERGO surface via π- π interaction. In the presence of Pb2+, Apt forms G-quadruplex, leading to the detachment of Apt from the surface of ERGO/GCE. So the conformational change caused by Pb2+ produces the change in electrochemical signal. Electrochemical measurements showed highly sensitive detection of Pb2+ ions with a liner range from 10-15~10-9 M and a detection limit of 0.51 fM. The sensor also has high selectivity of Pb2+ ions, high stability, reproducibility, and regeneration.

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