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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Separation of exosomes and lipoproteins in human serum using frit-inlet asymmetrical flow field-flow fractionation with multi-angle light scattering

2019년 8월 22일 12시 50분 53초
ANAL1.O-8 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 21분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Young Beom Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Exosomes play an important role in maintaining homeostasis and intercellular communication in body fluids and are known to be associated with physiological and pathological functions. Recently, exosomes are expected as a biomarker for disease prognosis and diagnosis. In particular, a number of studies have shown that blood exosomes are closely related to cancer. Lipoproteins carry lipids through the blood stream and are classified according to its size and density. Because of the similarity in their size and density of lipoproteins, it is important to separate exosomes and lipoproteins from blood Field-flow fractionation (FFF) is an analytical technique that is capable of separating nano to micron sized particles in an aqueous solution in an empty channel space without packing materials. In this study, exosomes and lipoproteins in human serum were separated by frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) using field programming and monitored by a series of UV and multi-angle light scattering (MALS) detectors. During separation, fractions of exosome and lipoprotein were collected and analyzed by Western blot, transmission electron microscope (TEM), and nUPLC-ESI-MS/MS for proteomic.