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제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 lipidomic analysis of serum from mice exposed to ambient particulate matter using LC-MS approach

2019년 8월 29일 15시 25분 59초
ANAL1.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 42분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Seoyoung Jang, Geum-Sook Hwang*
Western Seoul Center, Korea Basic Science Institute, Korea
Ambient particulate matter(PM) has known as environmental risk factors in Korea, and associated with disease including asthma, cardiovascular and nervous disorder. However, the molecular mechanism by exposure to PM has not been fully understood.. We observe the metabolic perturbations in response to PM with a diameter 2.5-10 µm (PM10) exposure in mouse model. In this study, C57BI/6J mice (male) were exposed to PM10 or clean air for 3 weeks in chambers. We conducted untargeted lipidomic analysis of serum from mice to evaluate metabolic perturbations using ultra performance liquid chromatography/quadrupole time of flight mass spectrometry (UPLC/Q TOF MS). Partial least squares - discriminant analysis (PLS-DA) score plots showed a clear separation between the PM10 exposed group and control group. We observed changes in lipids including ceramide, free fatty acid, acylcarnitine, phospholipid and diacylglycerol species in PM10 exposed mice compared to control. phospholipid species were decreased in PM10 exposed group compared to control, whereasacylcarnitine and free fatty acid species were significantly increased in PM10 exposed group, indicating the alteration in fatty acid oxidation metabolism. This study demonstrates that the LC-MS based-lipid profiling can be a used to understand the metabolism of PM10 exposed group on biological system.