KCS

Biotechnology products and medicines based on stem cells are being developed in variety. The development of stem cell bioproducts and therapies require a large amount of stem cells. In order to develop such products, it is necessary to process a culture system capable of mass production of stem cells. We carried out experiments with the aim of developing technology to monitor stem cell culture markers through quantitative analysis of metabolites and proteins related to cell growth and proliferation. “Additive A” was inserted into the control media to develop a medium in which the proliferation rate of stem cells was fast and the morphology of the cells were not altered. The mesenchymal stem cells were cultured for 5 time points (6 hours, 1 day, 2 days, 3 days, and 4 days) in the new media which is added Additive A and the control media. As a result, it was found that glutamine and sugar were consumed more in the new media than in the control media, and this shows that the cells of the new media required more energy which is needed for cell proliferation. Proteome quantification was performed by clustering the ratio of the control media to the new media according to the time points. As a result, we observed biological processes and KEGG pathways related to Additive A in the cluster which show a decreasing pattern over time. We conclude that Additive A is involved or helps in cell proliferation.