abs
본문내용바로가기
KCS
학술발표회
화학이야기
발행지
학회소식
학회소개
KR
EN
학술발표회
상세안내
초록보기
초록통계
초록집 모음
초록등록
기기전시회 안내
화학이야기
주기율표
화학술어
화합물 명명법
네이버 화학백과
연구실험실 안전정보
연구윤리 정보
화학올림피아드
화학포스터 시화대회
미래화학자 연구발표회
발행지
저널
화학세계
화학교육
학회소식
학회소식/공지사항
학술행사/정보마당
언론에 비친 KCS
구인/구직
회원동정
후원사 혜택 안내
지면 광고 안내
공익법인 기부금 내역서
학회소개
정관/세칙
소개 및 활동/사업
현임원
역대임원
연혁
역대 학회상 수상자
대한화학회 창립 75주년
분과회/지부
학회자료실
이사회 회의록
단체회원
전자앨범
사무국/오시는 길
로그인
회원가입
회원안내
로그인
회원가입
회원안내
English
메뉴열기/닫기
학술발표회
초록보기
홈
학술발표회
학술발표회
화학이야기
발행지
학회소식
학회소개
초록보기
상세안내
초록보기
초록통계
초록집 모음
초록등록
기기전시회 안내
초록문의
abstract@kcsnet.or.kr
결제문의
member@kcsnet.or.kr
현재 가능한 작업은 아래와 같습니다.
09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능
제124회 대한화학회 학술발표회, 총회 및 기기전시회
Effective Sample Preparation of Polyphenols in Wine using Deep Eutectic Solvent-based Dispersive Liquid-Liquid Microextraction for HPLC-UV Determination
등록일
2019년 8월 22일 11시 18분 40초
접수번호
3105
발표코드
ANAL2.O-8
이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 09시 : 21분
발표형식
구두발표
발표분야
Analytical Chemistry -
Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Jongsung Noh
, JinSol Lee
, Seung Hoon Song
, Won Hoe Koo
, Hyun-Woo Cho
1
, Seung Woon Myung
*
Department of Chemistry, Kyonggi University, Korea
1
Department of Natural Science Chemistry, Kyonggi University, Korea
Polyphenols are phytochemicals that exist in grapes and are beneficial to human health. In this study, resveratrol, oxyresveratrol and piceatannol in wine were extracted by deep eutectic solvent based on dispersive liquid-liquid microextraction (DES-DLLME), and a method for quantification these polyphenols using high performance piquid chromatograph - UV/Vis (HPLC-UV/Vis) was established. Several parameters for sample extraction, clean-up and concentration were optimized and verified by Central Composite Design (CCD) using Design Expert 11. The optimized sample preparation parameters are as follows: type and ratio of DES as extraction solvent, methyltributylammonium chloride / decanoic acid(1:3 molar ratio); type and volume of basic solvent, KHCO
3
and 1.3 mL; volume of acetic, anhydride, 250 μL; time of derivatization, 5min; type of dispersive solvent, methanol; ratio of extraction and dispersive solvent, 1:5.5; and salt 1.0 g. Chromatographic separation with HPLC/UV-vis was performed using Agilent HPLC 1100 series, ACME C
18
(4.6mm id × 150 mm length, 5 μm particle size) column and gradient elution mode using water and 70% methanol. From the established extraction and HPLC-UV conditions, the limit of detection (LOD) and limit of quantitation (LOQ) of three analytes in the spiked sample were 6.09 - 11.77 μg/L and 18.47 - 35.66 μg/L, respectively. Recovery studies were performed at low, medium and high concentration range in calibration curve, and accuracy and precision in working range were 81.33 - 108.25 % and 2.03 - 10.73 RSD %, respectively. The calibration curves for the quantitative analysis were obtained the concentration range of 18.47 - 184.7 μg/L, 35.66 - 356.6 μg/L and 27.42 - 274.2 μg/L with correlation coefficient(r
2
) from 0.9909 to 0.9984, respectively. The proposed method was applied to the determination of polyphenols in wine samples.