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제124회 대한화학회 학술발표회, 총회 및 기기전시회 Effective Sample Preparation of Polyphenols in Wine using Deep Eutectic Solvent-based Dispersive Liquid-Liquid Microextraction for HPLC-UV Determination

2019년 8월 22일 11시 18분 40초
ANAL2.O-8 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 21분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Jongsung Noh, JinSol Lee, Seung Hoon Song, Won Hoe Koo, Hyun-Woo Cho1, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Department of Natural Science Chemistry, Kyonggi University, Korea
Polyphenols are phytochemicals that exist in grapes and are beneficial to human health. In this study, resveratrol, oxyresveratrol and piceatannol in wine were extracted by deep eutectic solvent based on dispersive liquid-liquid microextraction (DES-DLLME), and a method for quantification these polyphenols using high performance piquid chromatograph - UV/Vis (HPLC-UV/Vis) was established. Several parameters for sample extraction, clean-up and concentration were optimized and verified by Central Composite Design (CCD) using Design Expert 11. The optimized sample preparation parameters are as follows: type and ratio of DES as extraction solvent, methyltributylammonium chloride / decanoic acid(1:3 molar ratio); type and volume of basic solvent, KHCO3 and 1.3 mL; volume of acetic, anhydride, 250 μL; time of derivatization, 5min; type of dispersive solvent, methanol; ratio of extraction and dispersive solvent, 1:5.5; and salt 1.0 g. Chromatographic separation with HPLC/UV-vis was performed using Agilent HPLC 1100 series, ACME C18 (4.6mm id × 150 mm length, 5 μm particle size) column and gradient elution mode using water and 70% methanol. From the established extraction and HPLC-UV conditions, the limit of detection (LOD) and limit of quantitation (LOQ) of three analytes in the spiked sample were 6.09 - 11.77 μg/L and 18.47 - 35.66 μg/L, respectively. Recovery studies were performed at low, medium and high concentration range in calibration curve, and accuracy and precision in working range were 81.33 - 108.25 % and 2.03 - 10.73 RSD %, respectively. The calibration curves for the quantitative analysis were obtained the concentration range of 18.47 - 184.7 μg/L, 35.66 - 356.6 μg/L and 27.42 - 274.2 μg/L with correlation coefficient(r2) from 0.9909 to 0.9984, respectively. The proposed method was applied to the determination of polyphenols in wine samples.