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  • 09월 10일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Comparison of Solvent Effects on Cytotoxicity of Pt-based Drugs in 2D Cells and 3D Spheroid Cells

2019년 8월 28일 14시 20분 16초
ANAL2.O-18 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 51분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Min Ji Kim, Chae Eun Heo, Sooyeon Chae, Paul Valery Migisha Ntwali, Chae Ri Park, MyungKook Son, Da Gyeong Hyun, Hugh I. Kim*
Department of Chemistry, Korea University, Korea
Cisplatin (cis-[Pt(NH3)2Cl2]), a first-generation metal-based anticancer drug, has been widely used to treat diverse cancers including pediatric cancers. Cisplatin is commonly administered as a single drug or in combination with other drugs to raise its efficacy. Nevertheless, cancer cells could have acquired or intrinsic resistance to cisplatin. For this reason, a large number of in vitro studies have been conducted to clarify the mechanism of this phenomena. In most of these studies, dimethyl sulfoxide (DMSO) has been utilized as solvent for the stock solution of cisplatin and other drugs due to its ability to dissolve drugs in high concentration. According to a study concerning effects of solvents on the activity of cisplatin, DMSO depresses cytotoxic efficiency via ligand exchange with Cl-. To understand how DMSO directly impede platinum (Pt)-mediated toxicity, we employed 3 dimensional spheroids and 2 dimensional monolayer of SK-N-SH neuroblastoma cells to understand cisplatin activity in the presence of DMSO. Then we employed an inductively coupled plasma mass spectrometry (ICP-MS) for the quantitative analysis of cisplatin. Our quantitative results show that cellular uptake and DNA-Pt adduct formation is significantly reduced for the cells treated with cisplatin dissolved in DMSO compared to those treated with cisplatin dissolved in media. Overall, our study would provide valuable insight into the reduced efficacy of cisplatin for cancer treatment in the presence of DMSO, based on the correlation with structural change of cisplatin induced by DMSO.