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  • 09월 11일 00시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제124회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Cell Penetration of Multimeric Cationic Amphipathic Peptides at Nanomolar Concentrations

등록일
2019년 10월 1일 15시 51분 57초
접수번호
3226
발표코드
LIFE.O-7 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 10시 : 30분
발표형식
분과기념
발표분야
Life Chemistry - Oral Presentations by Young Life Chemists
저자 및
공동저자
Yan Lee
Division of Chemistry, Seoul National University, Korea
Cell penetrating peptide (CPP) is one of the effective ways to deliver difficultly penetrable molecules into cells. However, most CPPs show the penetrating activity at high micromolar concentrations, limiting their practical applications. We discovered that CPPs based on multimeric alpha-helical leucine (L) and lysine (K)-rich amphipathic peptides can effectively penetrate cells at 10 nM, a 1,000-fold lower concentration than the penetrable concentrations of previous reported cell penetrating peptides (CPPs), Tat or oligo arginine. The antiparallel conformation of the LK multimer was critical for the effective penetration of the CPPs at low nanomolar concentrations. The multimeric LK-CPPs show rapid cell penetration through macropinocytosis at low nanomolar concentrations, unlike the monomeric LK, which have slower penetrating kinetics at much higher concentrations. The heparan sulfate proteoglycan (HSPG) receptors are highly involved in the rapid internalization of multimeric LK-CPPs. As proof of concepts in biomedical applications, various biomolecules were delivered into cells with bioactivities at nanomolar concentrations. The multimeric CPP can be a useful platform for the intracellular delivery of biomacromolecular reagents that have difficulty with penetration in order to control biological reactions in cells at feasible concentrations for biomedical purposes.

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