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제125회 대한화학회 학술발표회 및 총회 Super-Resolution Microscopy Study of the Mechanism of Platelet Release

2020년 2월 4일 14시 23분 26초
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Physical Chemistry
저자 및
Seok Ran Go, Geun-ho Kim, Doory Kim*
Department of Chemistry, Hanyang University, Korea
Thrombocytopenia is a major clinical problem encountered across several conditions, including immune (idiopathic) thrombocytopenic purpura, chemotherapy-induced thrombocytopenia, aplastic anemia, and major cardiac surgery. Nevertheless, the terminal stages of proplatelet maturation and platelet release remain poorly understood. A better understanding of the cytoskeletal mechanisms that regulate platelet formation and determine platelet size may offer the promise of improved therapies for clinical disorders of platelet production and an important source of platelets for infusion. In this study, we resolved the three-dimensional ultrastructure of the actin and microtubule cytoskeleton in a platelet during its division process by using our custom-built STORM(STochastic Optical Reconstruction Microscopy) setup. We observed the microtubule and actin cytoskeleton twisting about the pre-platelet center several times which has not been observed with conventional microscopy techniques. We also use recently developed correlative STORM and electron microscopy and three-dimensional holography to quantitatively characterize nanoscale localization changes of organelles including endoplasmic reticulum, golgi apparatus and granules in a platelet during its division process. Our results highlight how different organelles are spatiotemporally organized in the different intermediate stages during the platelet division process.