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제125회 대한화학회 학술발표회 및 총회 Ultrastructural study of asymmetric differentiation process of stem cells

2020년 2월 4일 16시 30분 13초
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Physical Chemistry
저자 및
Jin Kyoung Chung, Geun-ho Kim, Doory Kim*
Department of Chemistry, Hanyang University, Korea
With the recent progress in super-resolution fluorescence microscopy technologies, the location and composition of proteins in a cellular event can be determined precisely, addressing many unanswered queries. The 2014 Nobel Prize in Chemistry for achieving nanoscale spatial resolution in optical imaging underscores the implication of optical nanoscopic imaging with super-resolution fluorescence microscopy. In this study, we applied this technique to investigate the comprehensive molecular mechanism of asymmetric differentiation of stem cells, which is a vital process in building a multi-cellular organism. We studied the differentiation of human dental pulp stem cells (DPSCs) to odontoblasts by using GPCR (G protein coupled receptor) inhibition, which is the excellent platform for studying asymmetric cell differentiation as the dramatic change in morphology from a fibroblast-looking cell to a neuron-like cell with a long single process can be achieved in a week. Applications of multi-color STORM (STochastic Optical Reconstruction Microscopy), correlative STORM and EM, and three-dimensional holography to the study of asymmetric differentiation of stem cells allow the location of different organelles to be determined with nanometer-scale spatial resolution and further provide novel insights into the fundamental principles of how human stem cells integrate the pathways controlling asymmetric differentiation, which are otherwise inaccessible by conventional methods.