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제125회 대한화학회 학술발표회 및 총회 Antifreezing Copolymers

2020년 2월 5일 18시 27분 00초
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Polymer Chemistry
저자 및
Jinkyung Park, Soyeon Kim, Zhengyu Piao, Byeongmoon Jeong*
Department of Chemistry and Nano Science, Ewha Womans University, Korea
The cryopreservation of cells, tissues and organs is essential for transplantation and medical advances in modern biotechnology. So far, Dimethyl sulfoxide(DMSO) has been widely used as a cryoprotectant although it shows intrinsic cytotoxicity. It can cause oxidative stress on the membrane of chondrocyte and affect the cell differentiation. Therefore, immediate removal of DMSO is necessary post thawing cells, but it is a time-consuming step which can lead to osmotic stress or loss of cells in its practical application. Here we reporting that the synthetic, biocompatible di-block copolymers, poly(ethylene glycol)-poly(L-alanine) (PEG-L-PA)s are able to inhibit ice recrystallization. In addition, the aqueous solution (5 wt.%) are excellent for cell recovery whereas over 10wt% DMSO is required for solvent based strategies. We also show that Ice Recrystallization Inhibition (IRI) activity and cell recovery after post thawing depend on each block length of PEG and PA. That results demonstrate amphipathic di-block copolymer can be alternative to toxic DMSO as a cryoprotective agent and have an important role in the storage and transplantation of biological material.