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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Enzymatic primer extension of unnatural nucleotide into DNA and its combination with Graphene Oxide for detection of miRNA21

등록일
2020년 9월 9일 10시 43분 39초
접수번호
0906
발표코드
LIFE.P-308 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 21일(수) 17:30~18:00
발표형식
포스터
발표분야
Life Chemistry
저자 및
공동저자
Moon Hyeok Choi, Young Jun Seo*
Department of Chemistry, Jeonbuk National University, Korea
Herein we designed new diagnostic method to detect miRNA21 via enzymatic primer extension of unnatural nucleotide with Morpholine naphthalimide deoxyuridine(dUrkTP) which is our research group have been developed. We tried to incorporate dUrkTP into DNA using DNA polymerase and separated dUrkTP including primer(rkDNA) using Lambda exonuclease and combined with Graphene Oxide(GO) for the detection of miRNA 21. rkDNA exhibited strong fluorescence but fluorescence was quenched in the presence of GO. This rkDNA-GO system showed enough selectivity and sensitivity for the detection of miRNA21 and it also exhibited short detection time that is around 30minutes. We believe that other types of miRNA also would be detectable by this probing system.

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