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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 DO-NCFC-RP/RPLC combined with FAIMS for comprehensive proteomics analysis

2020년 9월 10일 16시 59분 41초
ANAL.P-290 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 21일(수) 17:30~18:00
Analytical Chemistry
저자 및
Chaewon Kang, Dowoon Nam, Sang-Won Lee*
Department of Chemistry, Korea University, Korea
Human proteome analysis covering 10,000 proteins from a single cell type is possible, even if some methodological and technical problems remain. Identifying more than 10,000 proteins is still significant challenge, because identification efficiencies of present technology decrease under this condition. In this study, we introduce a dual online reverse-phase/reverse-phase liquid chromatography system based on an online non-contiguous fractionating and concatenating device (NCFC fractionator). In proteome analysis of complex human proteome, this system offers improved exploitation of separation space, so that a significant increase in the number of proteins identified in comparison with a common contiguous 2D-RP/RPLC method. In addition, the perfectly automated DO-NCFC-RP/RPLC system omits manual processes. As a result, this system minimizes sample loss and enables highly reproducible 2D-RP/RPLC experiments. Despite the advance in technology, a single-shot data dependent acquisition (DDA) is still limited to detection of complex human proteome. To increase the identification, we apply high-field asymmetric waveform ion mobility spectrometry (FAIMS) interface, which separate ions through differences in an ion’s mobility at low and high electric fields between inner and outer electrode. This interface selects ions pass from the ion source to the mass spectrometer for improved selectivity improved detection limits, and increased throughput. Here, we will discuss comparison between DDA data and FAIMS data.