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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Effects of ginsenoside Rb2 on osteogenic differentiation of C2C12 cells

2020년 9월 16일 17시 05분 11초
MEDI.P-434 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 21일(수) 17:30~18:00
Medicinal Chemistry
저자 및
Jae Wook Lee, Heesu Lee1, Seong-Hee Ko1,*
Convergence Research Center for Dementia DTC, Korea Institute of Science and Technology, Korea
1Department of Dentistry, Gangneung-Wonju National University, Korea
Objectives The most current therapies for osteoporosis have focused on inhibiting bone resorption by osteoclasts. Although the conventional drugs have therapeutic benefits, they also have disadvantages such as breast cancer and osteonecrosis of jaw. The purpose of this study is to develop the new anabolic agents for treatment of osteoporosis that have fewer risks compared to conventional therapies. Panax Ginseng is one of the most commonly used herbal medicines. Most of the biological activities of ginseng are derived from main components, ginseng saponins (ginsenosides). To determine the effect of ginsenosides on bone formation, we examined the effect of ginsenosides Rb2(G-Rb2) on C2C12 cell proliferation and osteogenic differentiation. Methods For determining the effects of ginsenosides on proliferation of C2C12 cells, we did MTT assay of C2C12 cells with ginsenoside Rb1, Rb2, Rc, Rd, Rg1 and Rg3 at 0.5, 5 and 50 μM . To evaluate whether ginsenoside Rb2(G-Rb2) could promote the osteogenic differentiation of C2C12 cells, nonosteogenic precursors, mRNA expression of runt-related transcription factor-2 (Runx2) was determined. mRNA of Runx2 expression was analyzed by quantitative real time PCR. And we examined the effects of G-Rb2 on mRNA expression of estrogen receptor α and β. Results G- Rb1, Rb2, Rc and Rd significantly increased C2C12 cell proliferation. G-Rg3 decreased C2C12 cell proliferation at 5, 50 μM. G-Rb2 significantly increased mRNA expression of Runx2 after 24 hours culture in osteogenic media . In addition, G-Rb2 increased mRNA expression estrogen receptor α and β both. However G-Rb2 upregulated expression of estrogen receptor β to a greater extent than that of estrogen receptor α. Conclusion These results suggest that G-Rb2 may promote osteogenic differentiation of osteoblasts and may have bone protective effect through estrogen receptor upregulation.