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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Specific detection of Zika and dengue viruses with graphene oxide-based biosensor

2020년 9월 17일 10시 51분 48초
MAT.O-5 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
화 10시 : 00분
Materials Chemistry - Oral Presentation for Young Material Chemists
저자 및
Ji-Seon Lee, Dal-Hee Min*
Department of Chemistry, Seoul National University, Korea
Due to the global warming and climate change, spread of flavivirus which is transmitted by mosquito vectors has threatened the human worldwide. Zika and dengue viruses are closely related flaviviruses which have a similarity in genetic information. It has been reported that co-infection between those viruses cause the severe diseases. Thus, rapid and accurate diagnosis of each virus (ZIKV and DENV S1-S4) is crucial to prevent the risks from repeated virus infections. Here, we report a graphene oxide-based molecular diagnostic strategy for multiplexed detection of relevant flaviviruses, ZIKV and DENVs by targeting viral genome. Specifically, we combined the PNA/GO-based biosensor with loop-mediated isothermal amplification (LAMP). During the amplification of viral RNA, single-stranded loops of the virus amplicon is produced, and fluorescent PNA probes can recognize them through target-probe hybridization. Meanwhile, graphene oxide can selectively distinguish and quench the fluorescence of unbound PNA driven by its preferential interaction with single-stranded nucleic acid over double-stranded one. This strategy facilitates differential diagnosis of similar viruses with distinctive specificity, and shorten the manual step of nucleic acid testing (NAT) by integrating the RNA extraction and amplification process of virus sample.