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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Symmetry-guided evolution of de novo metallo-β-lactamases

등록일
2020년 9월 17일 16시 09분 11초
접수번호
1165
발표코드
INOR.P-174 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 21일(수) 17:30~18:00
발표형식
포스터
발표분야
Inorganic Chemistry
저자 및
공동저자
Jaeseung Yu, Woon Ju Song1,*
Division of Chemistry, Seoul National University, Korea
1Department of Chemistry, Seoul National University, Korea
Directed evolution has yielded numerous novel bio-catalysts by mimicking natural evolution process in the laboratory. However, the adequate selection of evolution targets and the effective construction and screening of the mutant libraries still remain elusive. We herein suggested that protein symmetry can serve as a novel criterion to seek promising hotspots for protein evolution. We carried out iterative saturation mutagenesis of four symmetry-related residues located at rotation axes of de novo metallo-β-lactamase. The optimization of symmetry-related residues provided a significant effect in catalytic activities, while those of proximal residues exhibited trivial alterations. The identification and in vitro characterization of positive hits suggested that symmetry-guided evolution can yield considerable alterations in overall structures, metal-coordination geometry, and chemical environments of the active sites. Iterative evolution during four rounds produced high catalytic enhancement from the parent protein.

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