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제126회 대한화학회 학술발표회 및 총회 Amplification-free detection of COVID-19 target genes using a SERS-based microdroplet chip

등록일
2020년 9월 1일 18시 27분 00초
접수번호
1271
발표코드
ANAL2.O-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
화 10시 : 00분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Sohyun Park, Jaebum Choo1,*
Chemistry, Chung-Ang University, Korea
1Department of Chemistry, Chung-Ang University, Korea
The coronavirus disease 2019 (COVID-19) outbreak has been labelled an ongoing pandemic by the World Health Organization (WHO). Real time quantitative polymerase chain reaction (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a surface-enhanced Raman scattering (SERS)-based DNA detection platform for the rapid and sensitive detection of target DNA markers of corona virus. Just like the RT-qPCR, two DNA probes, a broad-range probe for E gene and an additional probe for RdRp gene, have been used for the simultanelous detection two target DNAs of corona virus. The Raman signals of DNA markers were measured under flowing conditions. Total analysis time from droplet generation to SERS detection takes less than 10 min because all experimental conditions are automatically controlled inside the exquisitely designed microfluidic channel. This novel SERS-based DNA sensing technology in a microdroplet channel is expected to be a powerful analytical tool to simultaneously detect multiple DNA oligomer markers of infectious disease.

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