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  • 09월 20일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제126회 대한화학회 학술발표회 및 총회 Size sorting of extracellular vesicles from cell using frit-inlet asymmetrical flow field-flow fractionation with multi-angle light scattering

등록일
2020년 9월 1일 13시 03분 56초
접수번호
1272
발표코드
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발표시간
화 10시 : 04분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Young Beom Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Extracellular vesicles (EVs) are cell-derived membrane-covered particles containing proteins, lipids, nucleic acids and other biological molecules. EVs play an essential role in the communication between cells via transporting pathologically important molecules. As the pathological importance of EVs increases, the expectation of EVs as a biomarker is growing. Subpopulations of EVs are divided into exosomes (30-100 nm in diameter) and microvesicles (100-1000 nm) according to their sizes and therefore, it is important to separate them because they differ in cellular origins, contents, and lipid compositions. However, it is challenging to isolate EVs while each of the separation methods such as ultracentrifugation, size exclusion chromatography, filtration, and precipitation has its own limitation. Field-flow fractionation (FFF) is a separation method capable of fractionating particles from nano to micron size in aqueous solution without using packing materials. In this study, exosomes and microvesicles secreted from cells were separated by frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) with field programming and monitored by UV and multi-angle light scattering (MALS) detectors. Eluted exosomes and microvesicles were identified by western blotting and their sizes were determined by MALS and dynamic light scattering (DLS). Further, lipidomic analysis was performed by nanoflow ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry to investigate the difference in lipid compositions between exosomes and microvesicles.

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