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제126회 대한화학회 학술발표회 및 총회 Microfluidic analytical device for Nucleic Acids Amplification and Detection

등록일
2020년 9월 1일 23시 06분 53초
접수번호
1273
발표코드
ANAL2.O-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
화 10시 : 08분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Nguyet mai Ly, Veasna Soum, Nayoon Pyun, Oh-Sun Kwon, Kwanwoo Shin*
Department of Chemistry, Sogang University, Korea
Polymerase chain reaction (PCR) for nucleic acid amplification plays an important role in genetics study as well as practical applications in diagnosis and detection, quality control, and quantitative monitoring of diseases, pathogens, or potential biomarkers. Conventional PCR is limited by time, equipment, tools, and skills of technicians. Many research groups have aimed to miniaturize PCR into a lab-on-chip device for point-of-care testing (POCT). Traditional approach is to use heating blocks with microfluidic device on which the flow is controlled by a supportive pumping system. To avoid dependence on pump, here we plan to build up a pumpless PCR chip controlled by capillary flow for self-regulated liquid delivery. Additionally, the flexible printed heaters on polyimide film fabricated by inkjet printer using conductive nano-silver ink have been provided for the underneath heating bed. For simple detection and easy visualization of amplified PCR products, a lateral flow assay will be employed later. This comprehensive device will promise a portable platform for POCT, being capable to complete both amplification and detection of target nucleic acid.

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